The Human Protein Atlas

Highly multiplexed dataset from normal/cancer TMA

This highly multiplex immunofluorescence dataset was generated using 46-oligonucleotide-barcoded antibodies (pre-conjugated antibodies by the vendor Akoya Biosciences) in the PhenoCycler Fusion system. The antibody panel was applied to Tissue MicroArray samples from normal human tissues on microscope slides. We followed the tissue sample preparation protocol described by Danielsson et al. (1). Tissue samples were handled in accordance with Swedish laws and regulations and obtained from the Department of Pathology, Uppsala University Hospital, Sweden, as part of the sample collection governed by the Uppsala Biobank (http://www.uppsalabiobank.uu.se/en/). All human tissue samples used were anonymized in accordance with the approval and advisory report from the Uppsala Ethical Review Board (Dnr Ups 02-577). TMAs were generated in accordance with strategies used in the Human Protein Atlas and as previously described (2, 3).

  1. Angelika Danielsson, Fredrik Pontén, Linn Fagerberg, Björn M Hallström, Jochen M Schwenk, Mathias Uhlén, Olle Korsgren, and Cecilia Lindskog. The human pancreas proteome defined by transcriptomics and antibody-based profiling. PLoS One, 9(12):e115421,2014.
  2. Mathias Uhlen, Per Oksvold, Linn Fagerberg, Emma Lundberg, Kalle Jonasson, Mattias Forsberg, Martin Zwahlen, Caroline Kampf, Kenneth Wester, Sophia Hober, et al. Towards a knowledge-based human protein atlas. Nature biotechnology, 28(12):1248–1250, 2010.
  3. Production of tissue microarrays, immunohistochemistry staining and digitalization within the human protein atlas. JoVE (Journal of Visualized Experiments), (63):e3620, 2012.

Dataset access

Please find this dataset publicly available at the BioImage Archive

Viewer

As a convenient way to explore the images we provide you links to each multiplexed core of the TMAs, powered by our in-house ellviewer code

TMA G

Stomach 1 core 3

Stomach 1 core 4

Stomach 2 core 1

Stomach 2 core 2

Stomach 2 core 3

Oral tissue core 1

Oral tissue core 2

Oral tissue core 3

Appendix core 3

Appendix core 4

Small intestine core 1

Small intestine core 2

Small intestine core 3

Duodenum core 1

Duodenum core 2

Duodenum core 3

Pancreas core 3

Pancreas core 4

Rectum core 1

Rectum core 2

Rectum core 3

Colon core 1

Colon core 2

Colon core 3

Lung core 3

Lung core 4

Gall bladder core 1

Gall bladder core 2

Gall bladder core 3

Liver core 1

Liver core 2

Liver core 3

Prostate core 3

Prostate core 4

Bladder core 1

Bladder core 2

Bladder core 3

Kidney core 1

Kidney core 2

Kidney core 3

Epididymis core 3

Epididymis core 4

Testis core 1

Testis core 2

Testis core 3

Seminal vesicle core 1

Seminal vesicle core 2

Seminal vesicle core 3

Placenta core 3

Placenta core 4

Ovary core 1

Ovary core 2

Ovary core 3

Fallopian tube core 1

Fallopian tube core 2

Fallopian tube core 3

Parathyroid core 4

Tonsil core 1

Tonsil core 2

Tonsil core 3

Lymph_node core 1

Lymph_node core 2

Lymph_node core 3

 

TMA H

Salivary gland core 1

Salivary gland core 2

Salivary gland core 3

Esophagus core 1

Esophagus core 2

Esophagus core 3

Skin core 1

Skin core 2

Vagina core 1

Vagina core 2

Vagina core 3

Nasopharynx core 1

Nasopharynx core 2

Nasopharynx core 3

Bronchus core 1

Bronchus core 2

Endometrium, post core 1

Endometrium, post core 2

Endometrium, post core 3

Endometrium, pre core 1

Endometrium, pre core 2

Endometrium, pre core 3

Cervix core 1

Cervix core 2

Breast core 1

Breast core 2

Breast core 3

Bone marrow core 1

Bone marrow core 2

Bone marrow core 3

Spleen core 1

Spleen core 2

Thyroid gland core 1

Thyroid gland core 2

Thyroid gland core 3

Adrenal gland core 1

Adrenal gland core 2

Adrenal gland core 3

Cortex core 1

Cortex core 2

Skeletal muscle core 1

Skeletal muscle core 2

Skeletal muscle core 3

Heart muscle core 1

Heart muscle core 2

Heart muscle core 3

Hippocampus core 1

Hippocampus core 2

Soft tissue 1 core 1

Soft tissue 1 core 2

Soft tissue 1 core 3

Smooth muscle core 1

Smooth muscle core 2

Smooth muscle core 3

Soft tissue 2 core 1

Soft tissue 2 core 2

Soft tissue 2 core 3

Cerebellum core 1

Cerebellum core 2